綠膿桿菌顯色培養基

HiFluoro Pseudomonas HiVeg Agar Base

原理

銅綠假單胞菌（又名綠膿桿菌）是革蘭氏陰性桿狀需氧菌。正如其他假單胞菌一樣，銅綠假單胞菌分泌幾種的色素，包括綠膿素（藍綠色）、熒光素（黃綠色，發熒光）、紅膿素（棕紅色）。King等人開發了假單胞菌瓊脂P（又稱King A培養基），用于提高綠膿素和紅膿素的產出，以及假單胞菌瓊脂F（又稱King B 培養基）用于提升熒光素的產出^【1】。

該顯色培養基用植物源蛋白胨取代動物蛋白胨，并基于King 等人的配方而成（熒光混合劑除外）。它是選擇性培養基，用于從膿液、痰和引流液等樣本中分離銅綠假單胞菌。

培養基中的溴棕*銨抑制其他菌群生長。它作為季銨類化合物和陽離子表面活性劑，使銅綠假單胞菌之外的細菌菌體釋放出氮和磷。銅綠假單胞菌裂解熒光底物，使UV下發射熒光。

培養基組成

配制

46.75 g溶于1000 ml蒸餾水（其中含10ml甘油）。加熱至沸騰使全部溶解，高壓滅菌。混勻和傾注平板。

培養反應

35-37°C培養18-24小時后，觀察如下：

參考文獻

1.King, Ward and Raney, 1954, J. Lab. Clin. Med., 44:301.

產品引用文獻

[1] BT Odumosu, BA Adeniyi, RC Dada-AdegbolaHannah - Blood, Multidrug resistant Pseudomonas aeruginosa from Southwest Nigeria hospitals. International Journal of Pharmaceutical Sciences Review and Research[J] , 2012,7:11-15.
[2] Seham, M. Shasha; Ahmad, Z. Al-Herrawyb,“Bactericidal activity of chlorine on some pathogenic bacterial strains isolated from Nile water”,Egyptian Journal of Environmental Research[J].2014,2:15-27.
[3] M. Ginovyan1, V. Hovsepyan3, M. Sargsyan 2, Antibiotic resistance of Pseudomonas species isolated from Armenian fish farms,Аквакультура[J].2017,11:163-173.
[4] K.Grigoryan G. Badalyan M .SargsyanA. Harutyunyan.,  LWT - Food Science and Technology[J].
2014, 58(10 ): 360-363

[5]BT Odumosu, BA Adeniyi, RC Dada-AdegbolaHannah - Blood, Multidrug resistant Pseudomonas aeruginosa from Southwest Nigeria hospitals. International Journal of Pharmaceutical Sciences Review and Research[J] , 2012,7:11-15.

[6]Seham, M. Shasha; Ahmad, Z. Al-Herrawyb,“Bactericidal activity of chlorine on some pathogenic bacterial strains isolated from Nile water”,Egyptian Journal of Environmental Research[J].2014,2:15-27.

[7]M. Ginovyan1, V. Hovsepyan3, M. Sargsyan 2, Antibiotic resistance of Pseudomonas species isolated from Armenian fish farms,Аквакультура[J].2017,11:163-173.

[8]K.Grigoryan G. Badalyan M .SargsyanA. Harutyunyan., LWT - Food Science and Technology[J].

2014, 58(10 ): 360-363